This is going to be short and sweet. The pictures explain a lot.
No guarentees on this grow and if you guys follow this log and it ends up not producing any (fruits) of my labor then I appologize.
First I made up some LC with spores from T.H.E. I used B+ and SA.
The LC colonized nicely and this is the first time Ive ever had a successful LC. It was made from 8 table spoons light karo and 900ml spring water
I rinsed my WBS until it ran clear. I put holes in bucket 1" from the top and every inch all the way around so WBS on the bottom could get rinsed nicely.
I soaked it for 24 hours, actually it was 48 bc I forgot about it. The water ended up being a rust color and bubbling like a frog was living in it. Very foamy too. Rinsed again until it ran clear. Strained for about 10 hours. 6 seems to be too little and makes the WBS too wet *espically the WBS on the bottom, and 24 is way too long and will take forever to colonize. I know from me previous grow.
I put the WBS into my mycobags and some in to jars... (luckly)
I PCed everything for 2 hrs at 15psi
I never let the PC depressurize on its own and never knew why people recommended it. I never had a problem doign it with jars but this is the reason why you need to for bags...
I got this nice syringe and stirred my jars
I sucked up some LC and innoced my bags
ALL of them got cobweb, I wish I had a picture of what it looked like... it was amazing and Im glad that I got it. The cobweb formed a PERFECT sphere on top of the WBS in the mycobags and there were spikes of cobweb comming straight out of the ball. AMAZING!
I innoced my jars using the multi spore technique.
I innoced more myco bags with the LC and it contamed AGAIN, this time so the the bags had green in the and some of them had black. There were little black dots ontop of the WBS in the bags. I think it was trich since it can come in many forms.
After my $25 investment for the bags was a complete failure I still had the jars to work with (luckly
) During the colonization time of the jars I shook them 6 times... foolish.
One of the jars appreared to have a worm in it and I know that you CAN get flies
I guess it was just a stick in the WBS. Nothing to worry about.
They took over a month to "fully" colonize.
The jars were like this for 2 weeks with no progress. Many people said it was a bacteria infection and this and that but I asure you it was nothing of the sort.
I finally got tired of waiting around I figured once I spawn to bulk with the WBS being so close to being fully colonized that it will just finish in the monotub.
I read a tek about pasteurizing using a wallpaper steamer. It looks like it would work great but the site that had them refurbished for $20 no longer carries them...WTF?! TAKE THEM OFF THE SITE IF YOU ARENT GOING TO CARRY THEM ANYMORE...idiots.
I planed on pasteurizing in a cooler but putting 170 deg F water it with the poo in oven bags. Well, not oven bags but seal-a-meal bags. I made this little set up.
We had heavy rain for about a week straight and I put my poo outside on a shower curtian and let it get wet then dry in the sun then wet then dry. I took the dry poo and put it in to a 5gal bucket and put water in it little by little so it didnt get over saturated. We're looking for field consistancy right?
After I had that done I put the poo into bags. I didnt have a scale to weigh out how much I was using so I just guessed...its no big D. I put the poo into my pasteurizer.
I poured in the 170deg F water and closed the lid. I inside of the bags got to about 100 deg F and no higher. I put a small space heater right in from of the cooler to try to heat it up more. The bags then got to 110 deg F but still not enough. I took the water out of the cooler and put it back into my PC being used as a pot and heated the water again. This time to 210deg F. I poured it back in and it still wasn't hot enough.
By this time it was 4 in the AM and my girlfriend was passed out. So I got to use the stove :rofl:
The internal bag temp stayed about 150 for 1 hour. I took the bags out of the oven after checking all their temps and impulse sealed the bags. I knew that thing would come in handy for something else since I wont be doing bags again and my first set contaminated. I got a sealer
for $30 off a yahoo store. GREAT DEAL. Brand new with 2 extra elements.
I sealed the bags and let them cool until the next day. I needed some sleep, :crazy2: I was losing my mind.
I spawned 3 quart jars to ever to bags poo. I wiped the tub down with 91% alcohol because I ran out of the 70 or 71% w/e it is. I mixed it all together very well and covered with a blanket and turned my small heater on to get the monotub internal temp up to 79-81 deg. I sat in the closet for an hour to get the temp right. I think my girlfriend thought I was jerkin it or something :thumbup:
I used the rest of the poo to spawn to in 3 pie plates to do a little experiment.
1st plate) Mixed
2nd plate) Spawn on bottom and poo on top
3rd plate) Poo and layer of spawn on top.
I threw them in the incubator at 81 deg F. Just a simple TIT
Cups in there so when I have 349071234 pounds of jars and spawn in there it doesnt sag and melt a whole in my tub or burn this mother down
The mixed one is taking off and the one with the spawn on the bottom is a close second. The one with it on top has no visible signs yet. Lame.
I have this little thing I put together...
The temp gage on the left is room temp and the one on the right is my digital heater for my TIT. Its only 74 in the picture. Thats when I set it up and was waiting for the water to heat up.
Here is the monotub as of Aug 4th. I have 2 monotubs going and both of them are looking the same. Any ideas on how to get clearer pictures? Maybe I wont use the flash next time. Will turning the lights on for a few minutes ever day start pinning? I have to have light when I check my jars and shit. I don't want to make trouble for myself
IDK when I stated this grow but its been too long (bc I shook the jars too much. I will NEVER shake another jar, not even at 25% colonization.)
Here is a picture of what I have running in the room 24/7
It captures and kill bacteria.
Anyhow... I will continue this log as soon as there is more progress. I will also be posting pictures of the pie plates.
So its been about a week or so and the monotubs are looking good. I think that the substrate pulled away from the monotub side a little bit but I'm not sure.
The pictures are not very good because of the flash bouncing off the clear plastic. I would take out some poly fill and snap a picture like that but its just not worth the risk to me.
Metabolites in monotub 1
Metabolites in pie plate 2 (spawn on bottom)
So today I needed to see how far the monotubs have actually gone. Looking through the clear plastic in the dark is hard to tell. So I opened it... probably a mistake but we'll see
Last night I mixed up my casing mix. Its 50/50
The perfect mix is based on peat moss and horticulture perlite. I originally made it a 1:4 ratio with the 1 being the perfect mix but after reading the ingrediants I just made it 50/50.
With so much debate on whether to pasteurize or sterilize a casing mix I forgot what I was doing and sterilized it after promising myself to pasteurize. I ended up using all my vermiculite so I had to buy more and the sterilized casing mix Im going to use on my pie plates. I think I'm going to mix up some more 50/50 and PASTEURIZE it to use in my monotubs.
Monotub 1 bottom
Monotub 2 bottom
Going to be getting the internal temp to about 80 for the next 2 days too see if I can get these suckers colonized to 100% so I can case them.
Monotub 2 has a big crack in it... I think both of the tubs are DRY. I might dunk before I case.
Cased my pie plate tonight... the mixed one.
The monotubs are going slow. They colonized 90% quick as shit but its just not starting to get nice and thick in a few spots on top.
The pieplate casing is doing nothing yet.
The pie plate with spawn on top is just starting to break through the bottom
The pie plate with spawn on the bottom is at 100% and will be cased tomorrow.
The buckets are going nice, crazy rhizos on them.
My next set of jars are almost 100%
I need more jars to innoc with my 7 new strains