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  #1  
Old 09-21-2007, 07:40 PM
HMS Beagle
 
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Default Hydrogen Peroxide

Can anyone here confirm the rumor that a 1:20 dillution of hydrogen
peroxide can keep out contams while leaving the mycelium alive?

I assume this would have to be done well after initial growth inside
of the casing.
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  #2  
Old 09-21-2007, 07:40 PM
Yachaj Paye
 
Posts: n/a
Default Re: Hydrogen Peroxide

HMS Beagle schreef:
> Can anyone here confirm the rumor that a 1:20 dillution of hydrogen
> peroxide can keep out contams while leaving the mycelium alive?


Confirmed.

>
> I assume this would have to be done well after initial growth inside
> of the casing.


While it is true that many people add diluted hydrogen peroxide into the
casing layer I doubt if that really protects against contamination with
other mycelia (molds). The white mycelium produces exo-enzymes which
break down the peroxide pretty fast. A large amount of mycelium produces
a lot of those enzymes.

Peroxide works best when it has to protect a relatively large amount of
sterile substrate against contamination, i.e. when you have just added
some mycelial tissue to agar, grainspawn or bulk substrate. In the case
of a casing there is a lot of enzyme-producing mycelium and a little
amount of uncolonized material which needs to be protected.

Peroxide merely prevents the germination of unicellular contaminants
(mold spores, endospores, bacteria which haven't yet formed a colony
etc.). It does not protect in situations where a contamination enters in
the form of an established piece of enemy mycelium or a bacterial
colony. So when an insect lands on you casing with a piece of mold on
its legs or so peroxide won't help.

The best protection of a casing are probably bacteria which do not harm
the large amount of established mycelium but at the same time keep other
molds out.

Most important is to prevent the casing from drying out (molds love dry
casings). And never spray water on the casing (the thinnest layer of
water on the casing blocks the only possibility of the mycelium to
breathe fresh oxygen). And many not-cubensis mushrooms require a low
carbon dioxide/high oxygen environment. They won't fruit in a closed bag
or -plastic box.

The application of the aquarium bubbler technique can be very useful.

Yachaj Paye
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  #3  
Old 09-21-2007, 07:40 PM
Yachaj Paye
 
Posts: n/a
Default Re: Hydrogen Peroxide

HMS Beagle schreef:
> Can anyone here confirm the rumor that a 1:20 dillution of hydrogen
> peroxide can keep out contams while leaving the mycelium alive?


Confirmed.

>
> I assume this would have to be done well after initial growth inside
> of the casing.


While it is true that many people add diluted hydrogen peroxide into the
casing layer I doubt if that really protects against contamination with
other mycelia (molds). The white mycelium produces exo-enzymes which
break down the peroxide pretty fast. A large amount of mycelium produces
a lot of those enzymes.

Peroxide works best when it has to protect a relatively large amount of
sterile substrate against contamination, i.e. when you have just added
some mycelial tissue to agar, grainspawn or bulk substrate. In the case
of a casing there is a lot of enzyme-producing mycelium and a little
amount of uncolonized material which needs to be protected.

Peroxide merely prevents the germination of unicellular contaminants
(mold spores, endospores, bacteria which haven't yet formed a colony
etc.). It does not protect in situations where a contamination enters in
the form of an established piece of enemy mycelium or a bacterial
colony. So when an insect lands on you casing with a piece of mold on
its legs or so peroxide won't help.

The best protection of a casing are probably bacteria which do not harm
the large amount of established mycelium but at the same time keep other
molds out.

Most important is to prevent the casing from drying out (molds love dry
casings). And never spray water on the casing (the thinnest layer of
water on the casing blocks the only possibility of the mycelium to
breathe fresh oxygen). And many not-cubensis mushrooms require a low
carbon dioxide/high oxygen environment. They won't fruit in a closed bag
or -plastic box.

The application of the aquarium bubbler technique can be very useful.

Yachaj Paye
Reply With Quote
  #4  
Old 09-21-2007, 07:40 PM
Yachaj Paye
 
Posts: n/a
Default Re: Hydrogen Peroxide

HMS Beagle schreef:
> Can anyone here confirm the rumor that a 1:20 dillution of hydrogen
> peroxide can keep out contams while leaving the mycelium alive?


Confirmed.

>
> I assume this would have to be done well after initial growth inside
> of the casing.


While it is true that many people add diluted hydrogen peroxide into the
casing layer I doubt if that really protects against contamination with
other mycelia (molds). The white mycelium produces exo-enzymes which
break down the peroxide pretty fast. A large amount of mycelium produces
a lot of those enzymes.

Peroxide works best when it has to protect a relatively large amount of
sterile substrate against contamination, i.e. when you have just added
some mycelial tissue to agar, grainspawn or bulk substrate. In the case
of a casing there is a lot of enzyme-producing mycelium and a little
amount of uncolonized material which needs to be protected.

Peroxide merely prevents the germination of unicellular contaminants
(mold spores, endospores, bacteria which haven't yet formed a colony
etc.). It does not protect in situations where a contamination enters in
the form of an established piece of enemy mycelium or a bacterial
colony. So when an insect lands on you casing with a piece of mold on
its legs or so peroxide won't help.

The best protection of a casing are probably bacteria which do not harm
the large amount of established mycelium but at the same time keep other
molds out.

Most important is to prevent the casing from drying out (molds love dry
casings). And never spray water on the casing (the thinnest layer of
water on the casing blocks the only possibility of the mycelium to
breathe fresh oxygen). And many not-cubensis mushrooms require a low
carbon dioxide/high oxygen environment. They won't fruit in a closed bag
or -plastic box.

The application of the aquarium bubbler technique can be very useful.

Yachaj Paye
Reply With Quote
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Psilocybin mushrooms possess psychedelic properties. They are commonly known as "magic mushrooms" or "shrooms", and are available in smart shops in many parts of the world, though some countries have outlawed their sale. A number of other mushrooms are eaten for their psychoactive effects, such as fly agaric, which is used for shamanic purposes by tribes in northeast Siberia. They have also been used in the West to potentiate, or increase, religious experiences. Because of their psychoactive properties, some mushrooms have played a role in native medicine, where they have been used to affect mental and physical healing, and to facilitate visionary states. One such ritual is the Velada ceremony. A representative figure of traditional mushroom use is the shaman and curandera (priest-healer) María Sabina. Medicinal mushrooms Currently, many species of mushrooms and fungi utilized as folk medicines for thousands of years are under intense study by ethnobotanists and medical researchers. Maitake, shiitake, and reishi are prominent among those being researched for their potential anti-cancer, anti-viral, and/or immunity-enhancement properties. Psilocybin, originally an extract of certain psychedelic mushrooms, is being studied for its ability to help people suffering from mental disease, such as obsessive-compulsive disorder. Minute amounts have been reported to stop cluster and migraine headache

Psilocybin mushrooms (also called psilocybian mushrooms) are fungi that contain the psychedelic substances psilocybin and psilocin, and occasionally other psychoactive tryptamines. There are multiple colloquial terms for psilocybin mushrooms, the most common being magic mushrooms or 'shrooms. And Psilocybe cubensis is a species of psychedelic mushroom whose principle active compounds are psilocybin and psilocin. Psilocybe cubensis belongs to the Strophariaceae family of fungi and was previously known as Stropharia cubensis. The mushroom's cap is reddish-cinnamon brown to golden brown in color with white to yellowish stipe and will turn bluish/greenish when bruised.