is this possible?

[Zeus]

can you cut a piece of mycelium from a colonized cake and drop it in a jar of grain and colonize that also?

[Fungus Maximus]

Its very possible, however for these kind of transfers it is better to use a liquid culture and inject through a self healing port, or to grow the spores on agar and do a sterile transfer to the grain jar, but if you dont have agar, and dont want to make LC, then you can certainly do what you are asking, it just needs to be done in a sterile environment, and the cake must be free of contaminates, basicaly you must have filtered air going to the cake at all times as it needs to be sterile to work.

[Zeus]

whats a liqiud culture?

[AnotherDimension]

An LC is a Liquid Culture. It is basically 4-5% natuarl sugars or nutes, Karo syrup (light) is the simplest. 1 1/3 cup water and 3/4 tspoon karo. Mix and PC for 20min @ 15psi. You squirt a few cc's of spores in and it will grow suspended in the jar, then when you stir it up n suck it up with a needle you are injecting dikaryotic cells instead of monokaryotic (look it up cuz I can't explain it well) anyway dikaryotic growth is much faster so an LC lets you skip a step kinda, so it is liquid nutes and water with myc mixed in so when you noc up wbs (wild bird seed or grains) it colonizes a lot faster then spore solution. I usually take a cake and in a glove box crumble and mix well with the same amount of uncolonized brf or wbs and in 3 days they are both fully colonized and you can do it again soo within a short time one cake can becom 10 trays:D

[Zeus]

oh man thanks for that info guys

[Fungus Maximus]

No problem, glad to help, AD is awesome though.