View Full Version : Frozen Spores!!!
superyong
06-06-2007, 02:25 AM
Does anyone know if spores still work after being frozen?
As I've just gone to start a new Mycobag to find my spores have frozen at the back of the fridge !!
legallyhomeless
08-20-2007, 02:03 PM
They are dead
spanki
12-06-2007, 05:25 AM
I ordered them through spores 101 will they be ok because if they are dead I will be pissed and i was wondering if I order through ralph will they be good or should I order a print I am positive that they can wistand the cold temps
Mycrobia
02-05-2008, 06:18 AM
They are dead
How do you know they are dead?
Neotoma
02-05-2008, 04:09 PM
Spores from different vendors would not be more freeze resistant than others.
AnotherDimension
03-01-2008, 08:14 PM
Ya that is silly. It would depend on whether the cell burst from internal water expansion when frozen...plenty of molds can make it and Psil Azures and Cyans come out in the snow....I bet some dry spores on a print would be fine but you would probably be lucky if the ones in water were still good. Though there are millions so statistically a few should make it...lol
legallyhomeless
03-02-2008, 07:40 AM
I should have mentioned that if it was a print, it would be fine, but spores in water = dead. I had a print and a syringe in my car for about 2 months. It was exposed to 90+ deg F during the day and freezing temps at night. They syringe was toast, the print was viable.
Nitro72
04-17-2008, 06:15 PM
Spore prints can be kept in a freezer. I have kept texas spores for over 3 months and they were OK. But syringes? No! Ice crystals have sharp edges and would pierce the cell membranes of spores.
how log can i keep a print? with out a freezer or anything.
Nitro72
04-24-2008, 07:33 PM
If spore prints are kept cool and dry, they can be stored for years. It would probably not last that long in a freezer. I keep mine in a small baggies tucked away in tupperware container.
DJFool1011
04-28-2008, 07:55 PM
i have a print i just used was 5 and a half years old and still seem to work so far
Braniac
01-31-2009, 08:36 PM
i am bringing this up because it is cold outside....I have sent spore syringes all over the world and never had a complaint about frozen syringes. So if it worrying you about the weather do not let it! The syringes will be fine.
invitro explorer
02-16-2009, 05:40 AM
They are dead
This is complete bullshit. Frozen multi spore solution in a syringe is completely okay. The only problem you could run into with this is if the syringe itself was damaged during freezing/thawing. I've seen bursted syringes, and sometimes the plunger piece will get warped and contaminants sneak in. But the spores themselves are completely fine to use after being frozen.
Don't post misinformation for the simple sake of having a higher post count.
Legally homeless is quite the accomplished grower, but in the same respect just because you freeze something doesn't mean its always dead though I did loose a dozen syringes that were all useless after they froze in the back of my fridge and it was of the PFAX isolates spores so PFAX is no more, so you both are right. The new LAVA syringes from Earths tongue can withstand being frozen, the average syringe wont make it when frozen solid...
boltrigboiley
02-16-2009, 08:22 PM
hold on a minute i thought spores could travel through space and still be viable ( according to scientists ) and its fucking cold in space.
invitro explorer
02-16-2009, 10:41 PM
Legally homeless is quite the accomplished grower, but in the same respect just because you freeze something doesn't mean its always dead though I did loose a dozen syringes that were all useless after they froze in the back of my fridge and it was of the PFAX isolates spores so PFAX is no more, so you both are right. The new LAVA syringes from Earths tongue can withstand being frozen, the average syringe wont make it when frozen solid...
Fair enough. I received a promo pack from a vendor that I won't mention on here out of respect for that fact that you have a personal grievance with them. So I'm sure you know who I'm talking about, rofl. Anyway, they sat outside my door for three days, 8000 feet up on the Colorado front range. Temps were significantly below freezing the whole time. All 6 syringes resulted in healthy myc and went all the way to fruit. Like I said, the only problem I've EVER had with a frozen syringe is with the syringe itself, not the spores contained inside.
Thats awesome, I wish I could have been so lucky...PFAX were albino mutants and they were awesome lil trolls....
invitro explorer
02-17-2009, 11:41 PM
Thats awesome, I wish I could have been so lucky...PFAX were albino mutants and they were awesome lil trolls....
That is harsh. How did they perform? And how were they under the scope?
mycobabe
03-04-2011, 06:01 AM
I would not throw those spores out just yet... From a scientific standpoint... being my career... that is the whole job of a spore, other than reproduction, is to protect the "insides" from unfavorable conditions.... Actually, at work, we freeze everything we want to keep... bacteria, fungi...
mycobabe
05-10-2011, 02:59 AM
good chance they are lost.. sorry... next time you order spores .. try earths tongue.. i've heard good things about them
vBulletin® v3.6.8, Copyright ©2000-2012, Jelsoft Enterprises Ltd.